An alternative peptone preparation using <i>Hermetia illucens</i> (Black soldier fly) hydrolysis: process optimization and performance evaluation.

Response surface analysis This study revealed a notable trend: the hydrolysis degree, at a constant enzyme concentration, initially increased and later decreased as the temperature varied between 45 °C and 60 °C ( Fig. 1 ).

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As shown in Table 3 , the regression analysis demonstrated that A, B, A 2 , and B 2 were highly significant, as evidenced by P -values less than 0.01.

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