Mutating alfalfa <i>COUMARATE 3-HYDROXYLASE</i> using multiplex CRISPR/Cas9 leads to reduced lignin deposition and improved forage quality.

Highly significant (p < 0.001) elevation of IVTDMD was also recorded in mutant lines Msc3h-013 and Msc3h-158 with a relative advantage of 8% and 10% over EV, respectively ( Figure 4E , Supplementary Table S1 ), followed by Msc3h-121 with a relative advantage of 5% over EV, which was not statistically significant ( Figure 4E , Supplementary Table S1 ).

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