Compared with non-stimulated conditions, we observed a highly significant increase in the frequency of divided CD8 − T-blasts from healthy donors (mean: 74.7%, range [73–78.4%]) and patients (mean: 57.5%, range [39.8–70.9%]) following 5 days of stimulation with anti-CD2/CD3/CD28 beads ( Fig. 6 C ).
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