A green strategy to produce potential substitute resource for bear bile using engineered Saccharomyces cerevisiae.

Results of Box–Behnken design for incubation condition optimization The p values of variables A and B were less than 0.001 (Table 2 ), which means that factors A and B were extremely significant, indicating that both the incubation time and temperature have significant influence on the biotransformation of TUDCA, according to the principle that the smaller the p value, the more dominant the corresponding influencing factor.

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