Positive selection underlies repeated knockout of ORF8 in SARS-CoV-2 evolution.

While the effect sizes estimated are barely significant, power analysis identifies only 22% power to identify a significant effect for hospitalization and 75% power to find an effect for death.

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Combining both dN/dS estimates and cluster size ratios for each clade, we see that 6 clades are differentiated from other clades by their elevated measures for nonsense mutations in both metrics, even if they failed to achieve significance levels (Supplementary Fig.

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