Comparative transcriptome analysis between two different cadmium-accumulating genotypes of soybean (Glycine max) in response to cadmium stress.

The gene expression levels measured by RT-qPCR were compared to those measured by RNA-seq methods, the results showed a highly significant correlation (R 2 = 0.87) was observed between the RT-qPCR and RNA-seq data sets (Fig. 2 ), which confirmed that RNA-seq method generated reliable expression data.

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