Ultrasonication followed by aqueous two-phase system for extraction, on-site modification and isolation of microalgal starch with reduced digestibility.

For the starches in the bottom pellets, the elevation of ultrasonic power from 200 W to 300 W significantly reduced the RC from 27 % ∼ 30 % to 18 % ∼ 20 % ( p < 0.05), while the increase of ultrasonic duration showed no significant influence on the RC ( p > 0.05), although an increasing trend could be observed under both 200 W (USB200-10 to USB200-15) and 300 W (USB300-15 to USB300-25) ( Table 2 ).

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