highly significantp < 0.01
The shoulder widths of CA genotype sheep were significantly greater than those of CC genotype sheep ( Figure 3E ) ( p < 0.05), and the differences in rump width were highly significant ( p < 0.01), as shown in Figure 3D . 3.4 Prediction of nsSNP protein structure related to growth traits of Ujumqin sheep To better understand how the above two mutation sites affect the expression of genes involved in the growth trait changes of Ujumqin sheep, we used TBtools to extract the CDSs of genes before and after the mutation and used online software to predict the secondary structures of mRNA and the secondary structures and tertiary structures of encoded proteins.