Transcriptomic and metabolomic correlation analysis: effect of initial SO₂ addition on higher alcohol synthesis in <i>Saccharomyces cerevisiae</i> and identification of key regulatory genes.

For isobutanol, only the EC1118a -SER33 knockout strain showed a decreasing trend and the effect was significant with a reduction of 71.346%; whereas for isoamyl alcohol, the EC1118a -ADH4 and EC1118a -TIR1 knockout strains showed a significant reduction of 21.003 and 17.334%, respectively.

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Results We found that, as the SO 2 concentration increased, the production of total higher alcohols showed an overall trend of first increasing and then decreasing.

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