Differences between the groups were considered statistically significant when p < 0.05, very significant when p < 0.01, and extremely significant when p < 0.001. 2.6 The preparation and collection of single cells for gene expression profiling The mice were anesthetized with PTB (100 mg/kg, i.p.) and perfused transcardially with a cold (4 ± 1°C) isolation buffer ( Table 1 ).
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The deletion of AQP4 and TRPV4 affects astrocyte swelling/volume recovery in response to ischemia-mimicking pathologies.
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