Nitrogen-cycling microbial communities respond differently to nitrogen addition under two contrasting grassland soil types.

In the temperate steppe, significant or highly significant differences were observed in soil total P, organic carbon, and fast-acting P contents at different incubation times ( p < 0.05 or p < 0.01).

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Additionally, the sampling time significantly affected soil total N, total P, pH, ammonium N, fast-acting P, and organic N ( p < 0.05), showing an increasing trend followed by the decreasing trend peaking at 30 days.

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