This co-treatment significantly decreased ADM2 expression versus UK-5099 alone within the matrix, and also led to a marginally significant decrease ( p = 0.0516 ) within the bulge ( Fig 3D and S9A Fig in S1 File ), consistent with previous findings [ 35 ].
RNAScope reveals that MPC inhibition increases the expression of ADM2 and ATF4 transcripts in the hair follicle matrix and bulge To further investigate these findings in situ on human hair follicle tissue sections, we employed RNAScope to label ATF4 , and ADM2 (an ATF4 target gene which showed a highly significant and high fold change increase; padj = 1.87E-61; 3.56 log2 fold change) in hair follicles treated with UK-5099.