scHolography: a computational method for single-cell spatial neighborhood reconstruction and analysis.

Not only were the differences highly significant between each cell type (Mann Whitney Wilcoxon test, p < 2.22e − 16), but also the spatial order agreed with stereotypical tissue organization such that suprabasal cells were furthest away from smooth muscle cells, followed by basal cells and fibroblasts (Fig. 2 f).

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6 f) across the reconstructed dermis showed a decreasing trend in the distance between Dermal 1–4 and Dermal 4 cells and unique patterns for each marker, respectively, in line with the notion that papillary dermal cells are located at the upper dermis whereas reticular dermal cells are located at the lower dermis [ 27 , 31 ].

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