Protocol to study the photosynthetic response of maize to the CO<sub>2</sub>-temperature coupling effect at ear stage using a specialized designed gradient.

Examine rows a for H 2 OR and H 2 OS, which should be within ±0.5 μmol mol −1 ; if they are not within this range and show a decreasing trend, it is advisable to wait for an additional 10 min.

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