Moreover, we noted an obvious trend that d -serine selection greatly stimulated TvDAO editing, as 24 out of the 27 (88.9%) d -serine-resistant T 1 plants carried homozygous TvDAO mutations (Fig.
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A dual-function selection system enables positive selection of multigene CRISPR mutants and negative selection of <i>Cas9</i>-free progeny in <i>Arabidopsis</i>.
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