Rare but diverse off-target and somatic mutations found in field and greenhouse grown trees expressing CRISPR/Cas9.

When the association was analyzed using 500 kb genome windows, a highly significant and positive, but very weak, correlation was found; gene density explained only 3% of the variance in off-target site density ( Figure 2C ; r = 0.17, p < 0.0001). 3.2 Targeted sequencing depth To estimate the coverage depth obtained by the bait-capture targeted sequencing approach at off-target sites, we computed the depth of coverage at pre-filtered variant sites identified by Mutect2 ( Figure 3A ).

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