Changes in the rumen development, rumen fermentation, and rumen microbiota community in weaned calves during steviol glycosides treatment.

In contrast, the proportions of propionate ( p = 0.549) and butyrate ( p = 0.056) had no significant change ( Figure 3F ), and the proportion of butyrate ( p = 0.056) showed an increasing trend. 3.4 Effects of STE treatment on the rumen bacterial community richness and diversity in weaned calves To explore how the supplementation of STE altered ruminal fermentation, DNA was extracted from ruminal digesta, and 16S rRNA gene sequencing was performed to study the changes in ruminal microbiota.

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