Monitoring maternal rubicon knockdown eggs fertilized by sperm expressing the CD8-Venus plasma membrane marker, and stained to visualize the axoneme, detected a highly significant attenuation in sperm plasma membrane breakdown upon maternal rubicon knockdown, as compared with counterpart control eggs (Fig. 4b–d ).
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Egg multivesicular bodies elicit an LC3-associated phagocytosis-like pathway to degrade paternal mitochondria after fertilization.
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