After the normalization of total reads, a decreasing trend in 5′-U reads of 21–25 nt was observed in these Fgrdrp deletion mutants, with the most pronounced decrease in Δ Fgrdrp2 (decreased by about 80%), followed by Δ Fgrdrp1 (~70%) and Δ Fgrdrp5 (~32%) ( Fig. 7B ).
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RNA-dependent RNA polymerases regulate ascospore discharge through the exonic-sRNA-mediated RNAi pathway.
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