In females, the expression levels of the 3 genes were low or had a decreasing trend at 5–10 days after eclosion, followed by a significant increase at day 15, then significantly declined at day 20–25, but eventually increased again at 30 days ( Fig. 2A–C ).
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Functional studies of McSTE24, McCYP305a1, and McJHEH, three essential genes act in cantharidin biosynthesis in the blister beetle (Coleoptera: Meloidae).
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