Prolonged premature rupture of membranes with increased risk of infection is associated with gut accumulation of <i>Pseudomonas</i> from the environment.

However, observed species ( P = 0.0713) and Chao1 index ( P = 0.0706) showed an increasing trend in the PPROM group ( Fig. 1 A and B), suggesting that the α-diversity of the gut microbiota in PPROM infants shows trend of correlation with PROM.

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