Low temperature exposure influences nitrogen metabolism resulting in decreased Cry1Ac insecticidal endotoxin content in cotton seeds.

Consequently, low-temperature stress occurs more frequently in Chinese cotton production due to the high latitude of Xinjiang, with an increasing trend in the frequency and intensity of extreme low temperatures [ 33 ].

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However, it showed a significant positive correlation with GOT, GPT, and protease activity, and a highly significant negative correlation with free amino acid content.

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Under low temperatures, the Cry1Ac endotoxin content in boll shell, fiber, and seed decreased, and with the decrease in temperature, their content showed a decreasing trend (Table 1 ).

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