Parabrachial nucleus Vglut2 expressing neurons projection to the extended amygdala involved in the regulation of wakefulness during sevoflurane anesthesia in mice.

Nevertheless, we observed a positive trend in the increase of δ oscillations during the process.

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There was a decreasing trend in calcium activity expression as they entered early phase III of anesthesia, with males showing a significant decrease compared to females (males: −0.6856 ± 0.1939 vs. females: −0.2641 ± 0.1398, p = 0.0259, n = 9 per group) (Figure 5C ).

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