When LwCas13a protein was applied to the SHERLOCK system, the fluorescence intensity of the LwCas13a protein group started to show an increasing trend at 25 min, and the final fluorescence intensity of the LwCas13a protein experimental group was at a high level after 3 h of reaction, but the negative control group had not been producing fluorescence intensity.
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CRISPR/Cas13a-based genome editing for establishing the detection method of H9N2 subtype avian influenza virus.
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