From the result, trigonelline was one of the metabolites with highly significant differences, especially in the result of the raw coffee beans group and the light-roasted group, as illustrated in Fig. 8 (A).
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Matrix-assisted laser desorption/ionization mass spectrometry for the rapid and high throughput analysis of betaine and trigonelline in <i>Lycium chinense</i> Mill. and trigonelline in coffee.
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