Maximum likelihood estimators for colony-forming units.

For a single sample with initial volume V 0 which is represented by one count of colonies n k at one dilution d k (because only one dilution was measured, or because only one spot or one plate in a series was countable), statistical error of counts [presumably but not necessarily ( 22 , 23 ) Poisson] is inevitable, and pipetting error will contribute but may not be significant.

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