The Tma proteins appear to make no contribution to recycling and REI at native uORF3, as the uORF3-only reporter expression is unaffected by the tma20 ∆ tma64 ∆ mutations; whereas its conversion to uSt-st3 confers a modestly significant Tma-dependence for recycling and suppression of REI (Fig. 7B , cols. 5–8).
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Differential effects of 40S ribosome recycling factors on reinitiation at regulatory uORFs in GCN4 mRNA are not dictated by their roles in bulk 40S recycling.
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