a decreasing trendp = 0.076
Rumen pH had a decreasing trend ( p = 0.076), which showed a linearly downward trend ( p = 0.078); however, the other ruminal fermentation indexes were not significantly affected by the experimental treatments ( p > 0.05). 3.1.4. 16S rRNA Gene Analysis of Bacterial Communities Using next-generation sequencing technology, 2,546,495 reads across all samples, with an average of 79,401 per sample, were acquired.