highly significantP < 0.001
22 , 23 A comparison of median gene fold change between amyotrophic lateral sclerosis, Parkinson’s disease, primary lateral sclerosis and healthy controls using a Kruskal–Wallis analysis was highly significant for all eight miRNAs with H values ranging from 38 to 136 (critical value = 16, P < 0.001), suggesting that at least one of the disease groups was not from the same population.