Two proteins (EF‐Tu 1 and EF‐Tu 2, of nearly identical sequence) use three separate V ligations in their top brackets (all performed at top‐level between starting segments), and one (RF2) contains a V ligation followed by a poor thioester in its limiting path; these V ligations are likely to be significant yield bottlenecks.
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BracketMaker: Visualization and optimization of chemical protein synthesis.
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