extremely significantP < 0.001
Moreover, the relative mRNA expression level of GPR 40 supplemented with 8% SBP was superior to the CON treatment in colonic mucosa (linear, P = 0.004; quadratic, P = 0.015), the mRNA expression level of GPR 43 with supplemented 8% SBP was extremely significant (up-regulated by many orders of magnitude) compared to that of the CON treatment in colonic mucosa (linear, P < 0.001; quadratic, P < 0.001) ( Fig. 3 A–E). 3.11 Colonic microbiota The OTU of the colonic content of the piglets showed that there were 1426, 1704, 2160, 1322 and 1288 OTU from the CON, 2% SBP, 4% SBP, 6% SBP and 8% SBP treatments, respectively, totaling 7900 OTU, with 689 common OTU among treatments ( Fig. 4 A).