There is an obvious trend indicating that mutants with reduced transferase activity (Y144F, S114A, Q136E, Q136F, H194S, and A17S), as inferred from the large amount of unconsumed SECO after conversion ( Supporting Information Figure S4), tend to form more SMG.
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Kinetics of Secoisolariciresinol Glucosyltransferase LuUGT74S1 and Its Mutants.
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