Combining the Vaginal Microbiome and Serum Metabolome to Screen for Potential Biomarkers of Early Pregnancy in Cows.

Metabolic Pathway Marker Validation The concentrations of LTB4, PGF2α, and AA in the serum of cows in the non-pregnant and pregnant groups were examined by ELISA, and it was found that, compared with the non-pregnant group, there was a significant decrease in the concentration of LTB4 in the serum metabolites of cows in the pregnant group ( p < 0.05), a highly significant decrease in the concentration of PGF2α ( p < 0.01) and a non-significant difference in the change in the concentration of arachidonic acid, AA, in the pregnant group (NS) ( Figure 4 ). 3.4.

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