Optimization of Reverse Transcription Loop-Mediated Isothermal Amplification for In Situ Detection of SARS-CoV-2 in a Micro-Air-Filtration Device Format.

The factors that significantly improved the reaction time, BSA blocking and EDTA, were carried over to a three-level Box–Behnken DOE study as well as Tris-HCl and Tween 20, which did not show as significant in the factorial experiment but were included in all runs, so their absence may be significant.

also in 13,097 other papers