Transcriptome sequencing analysis of overexpressed SikCDPK1 in tobacco reveals mechanisms of cold stress response.

Plants treated with cold showed a decreasing trend in relative water content, while transgenic plants showed a greater difference.

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Statistical analysis The expression of snow lotus SikCDPK1 gene was calculated using the 2 −∆∆Ct method [ 19 ], and the experimental data were subjected to LSD (least significance difference) multiple ANOVA using the SPSS 18.0 software, with the test of significance set at p < 0.05 (significant level) and p < 0.01 (highly significant level), and GraphPad Prism 9.1 was used for graphing.

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