A Novel Biosynthetic Strategy for Ginsenoside Ro: Construction of a Metabolically Engineered <i>Saccharomyces cerevisiae</i> Strain Using a Newly Identified UGAT Gene from <i>Panax ginseng</i> as the Key Enzyme Gene and Optimization of Fermentation Conditions.

Additionally, the multiple quadratic regression equation between the factors and the response value is expressed as follows: Y = 255.98 + 13.24A + 7.09B − 15.73C − 3.98AB + 4.33AC − 6.01BC − 31.59A 2 − 15.24B 2 − 29.43C 2 The results indicate that the initial medium glucose concentration, shaking speed, and culture temperature have a highly significant impact on ginsenoside Ro yield ( p ≤ 0.01), and the interaction between shaking speed and culture temperature also has a highly significant impact on ginsenoside Ro yield ( p ≤ 0.01).

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The response surface plot ( Figure 3 F) shows that with an increase in shaking speed, the ginsenoside Ro yield shows an increasing trend, while with an increase in culture temperature, the ginsenoside Ro yield initially increases, then decreases.

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