Simulation of 69 microbial communities indicates sequencing depth and false positives are major drivers of bias in prokaryotic metagenome-assembled genome recovery.

Impact of sequencing depth on the number of MAG recovery From a sequencing depth of 10 million to 60 million reads, an increasing trend in the number of MAGs recovered was observed across all three pipelines, irrespective of the community profile ( Fig 1A and 1D ).

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