highly significantp < 0.001
The results revealed highly significant differences in CAI values ( p < 0.001) (Fig. 6 A), N C values ( p < 0.001) (Fig. 6 B), and ω ratios ( p < 0.001) (Fig. 6 C) between ancestral and extant sequences, suggesting distinct evolutionary dynamics in codon usage and selective pressures between the two groups.