According to the statistics of the number of DEGs, the number of DEGs between female and male PGCs was higher and increased obviously during development, but the number of DEGs between female and male PGCs at the same developmental time point was lower, showing an increasing trend ( Supplementary Figure S2A ).
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Comparison of primordial germ cell differences at different developmental time points in chickens.
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During further development of male PGCs we found that the decrease of cell adhesion and extracellular matrix interactions showed an obvious trend of enhancement (394 DEGs), and members of collagen family as well as integrin family were down-regulated to varying degrees similar to the development process before ( Figure 3B, 3D, 3F, 3H ).
** p<0.01, extremely significant difference).