Monitoring of activity-driven trafficking of endogenous synaptic proteins through proximity labeling.

However, our experiments quantifying total changes in synaptic cleft biotinylation with or without activity did not reveal significant differences, suggesting that the total amount of proteins exposed at the cleft after SV exocytosis may not be significant enough to increase the already dense proteome of the synaptic cleft, thus contributing only a relatively small percentage change that may be beyond detection using western blot.

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