a trend toward significanceP = 0.053
While the differences between the wild-type and mutant females did not fall below the threshold for statistical significance ( P < 0.05), there was a trend toward significance after 30 min ( P = 0.053) and 60 min ( P = 0.072). 60 min was required for a similar percentage of trpVa 1/2 females to be fertilized as after only 5 min for wild-type females.