A p -value is less than 0.01 indicated a highly significant difference in the expression level of the candidate gene between the parents, which is indicated by “**” in the result figure. qRT-PCR reaction system: 1 μL of cDNA template, 0.4 μL each of forward and reverse primers (10 μmol/L), 5 of μL SYBR qPCR Mix (Toyobo, Tokyo, Japan), and 3.2 μL of ddH 2 O briefly centrifuged to mix. qRT-PCR amplification program: 95 °C for 5 min, 95 °C for 10 s, 57 °C for 20 s, and 72 °C for 20 s, for a total of 40 cycles.
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Genetic Loci Mining and Candidate Gene Analysis for Determining Fatty Acid Composition in Rice.
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