The reason for choosing 14-day-old and 28-day-old mice for NEC models is that if younger mice were used, they were prone to death leading to experimental failure, if older mice were used, they do not meet the age at which NEC occurs, and if 21-day-old mice were used, the difference may not be significant due to the proximity of the day ages.
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Sodium formate induces development-dependent intestinal epithelial injury via necroptosis and apoptosis.
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