Immunofluorescence analysis showed a significant reduction in the percentage of immunoreactivity for GFAP ( Figures 4G, H ) and the percentage of immunoreactivity for β3-Tubulin showed a decreasing trend but no significant difference ( Figure 4I ), confirming that miR-185-5p LOF inhibited the differentiation of NPCs, especially their differentiation to astrocytes.
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<i>miR-185-5p</i> regulates the proliferation and differentiation of neural stem/progenitor cells.
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