Discovery of quality markers of <i>Meconopsis quintuplinervia</i> based on an absorption-based metabolite approach and rapid quantification using polarity-switching UPLC-QQQ-MS/MS.

A comparison between the sample collected at the highest altitude (MQ1) and the batches collected at the lowest altitudes (MQ8) revealed a decreasing trend in alkaloid content with increasing altitude; for example, the protopine content in MQ1 was 5.83 μg/g but was 13.14 μg/g in MQ8.

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In contrast, the flavonoid content exhibited an increasing trend with higher altitudes; for example, the isorhamnetin content in MQ1 was 10.48 μg/g but was 6.22 μg/g in MQ8.

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