A shorter linker in the bispecific antibody RmAb158-scFv8D3 improves TfR-mediated blood-brain barrier transcytosis in vitro.

At this high mTfR Pro119 coating density, which was chosen to promote bivalent binding, when possible, we could observe a slight trend of increased binding with increased linker lengths (Fig. 3 b, Supplementary Table S2 , S3, Figure S5). 8D3 demonstrated the strongest and scFv8D3-scFc the weakest binding to mTfR Pro119 , which was as expected being that 8D3 and scFv8D3-scFc were considered controls for bivalent and monovalent binding respectively.

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