Optimization of Ultrasound Extraction of Total Anthocyanin From <i>Berberis kaschgarica</i> Rupr. by Response Surface Methodology and Its Antihypertensive Effect.

The relationship between the test variables and the response variables (yield) was established using multiple regression analysis, resulting in the following secondary‐order polynomial equation: Y = − 56.61834 + 0.071432 X 1 + 0.81887 X 2 + 0.43497 X 3 + 115.5413 X 4 − 4.50 × 1.0 − 5 X 1 X 2 + 4.46 × 10 − 3 X 1 X 3 + 0.030250 X 1 X 4 − 7.74 × 10 − 3 X 2 X 3 + 0.27850 X 2 X 4 − 0.598 X 3 X 4 − 4.69 × 1.0 − 3 X 1 2 − 0.014878 X 2 2 − 5.88 × 1.0 − 4 X 3 2 − 74.4325 X 4 2 The analysis of variance (ANOVA) for the regression equation reveals that both linear and quadratic terms were highly significant ( p < 0.001) indicating the robustness of the model (Table 3 ).

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