Development of a Copro-RPA-CRISPR/Cas12a assay to detect Echinococcus granulosus nucleic acids isolated from canine feces using NaOH-based DNA extraction method.

SYBR Green real-time PCR detection, standard curve, and LOD We established and assessed the standard curve, revealing a highly significant relationship value (R 2 = 0.996), with a slope within the accepted criterion (-3.152) ( Fig 4 ).

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