highly significantP<0.001
Furthermore, there is a significant difference (P<0.01) between samples of different culture days (3 and 5 days) and a highly significant difference (P<0.001) between the culture at 3 and 7 days (supplementary data), which could be as a result of disturbance at the initial stage of the culture due to extracellular membrane adhesion protein synthesis [ 29 ].