Evaluating the efficiency of 16S-ITS-23S operon sequencing for species level resolution in microbial communities.

Sci Rep · 2025 · PMC11754871 · PMID 39843557

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marginally significantno p-value reported

The variation among the amplicons was found to be marginally significant for the ONT dataset and significant for the PacBio dataset at species level (ONT: p = 0.055, R 2 = 0.372; PacBio: p = 0.0002, R 2 = 0.395, refer Supplementary Table 2), with significant differences seen across both sequencing platforms at genus level (ONT: p = 9.999e-05, R 2 = 0.751; PacBio: p = 9.999e-05, R 2 = 0.579).

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